Fig. 3.

Metabolism of diethyldithocarbamate by CYP2E1. DDC (100 μM) was incubated with reconstituted CYP2E1 (1 pmol/μl) in the reconstituted system. The control and NADPH⁺ samples were incubated for 30 min at 28°C. Samples were then injected onto the HPLC column, eluted, and monitored as described under Materials and Methods. The control samples (A) exhibited two peaks in the UV spectrum absorbing at 244 and 323 nm and eluting as a single peak having an elution time at 29.5 min (B). The sample incubated with NADPH (C) gave a single major metabolite with a UV spectrum peak at 221.1 (C) and having an elution time of 27.8 min (D).