Fig. 4.

Deconvoluted mass spectra for the native CYP2E1 (A) and the inactivated CYP2E1 (B) after reaction with diethyldithocarbamate under turnover conditions. The inactivations were performed as described under Materials and Methods. Aliquots of the primary reaction mixture containing 50 pmol of CYP2E1 were loaded onto a C4 column, and the CYP2E1 samples were separated from the rest of the components in the reconstituted mixture as described under Materials and Methods. The molecular masses of the CYP2E1 samples were analyzed by ESI-LC/MS as described under Materials and Methods.