Fig. 3.

GST-MDA-7/IL-24 potently induces apoptosis in primary AML cells. A to E, leukemic blasts were isolated as described under Materials and Methods from the bone marrow of five patients with AML (FAB classification M2) and exposed to the designated concentrations of GST-MDA-7/IL-24 for 24 or 48 h, after which the extent of apoptosis was determined using Annexin V/PI staining assay. On the other hand, protein lysates were prepared from patient 3 and subjected to Western blot analysis to monitor caspase-3 and caspase-8 cleavage/activation and PARP degradation.