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. 2010 Dec;78(6):1096–1104. doi: 10.1124/mol.110.068007

Fig. 4.

Fig. 4.

GST-MDA-7/IL-24 induces a marked reduction in the clonogenic potential of primary AML blasts but is relatively sparing toward normal CD34+ progenitor cells. A, leukemic blasts isolated from patient 3 were plated in methylcellulose in the presence of the designated concentrations of GST-MDA-7/IL-24 for 14 days, after which L-CFUs were enumerated and expressed as a percentage relative to untreated cells. B, normal CD34+ cells isolated from three normal subjects (N#1–3) were exposed to GST-MDA-7/IL-24 (50 or 100 nM) for 48 h, after which cell death was determined by flow cytometry using the Annexin V staining assay. C, normal CD34+ cells (N#1–2) were plated in methylcellulose in the presence of the designated concentrations of GST-MDA-7/IL-24 for 8 days, after which CFU-GMs were enumerated and expressed as in A. Each sample was analyzed in triplicate; values represent the means ± S.D.