Skip to main content
. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Methods Mol Biol. 2010;613:1–23. doi: 10.1007/978-1-60327-418-0_1

Table 1.

Choice of Regeneration Solutions.

Type of Bond Acidic Basic Hydrophobic Ionic
Weak Strength pH > 2.5 Formic acid HCl 10 mM Glycine/HCl pH < 9 10 mM HEPES/NaOH pH < 9 50% ethylene glycol 1 M NaCl
Intermediate Strength pH 2~2.5 Formic acid HCl 10 mM Glycine/HCl H3PO4 pH 9~10 NaOH 10 mM Glycine/NaOH pH 9~10 50% ethylene glycol 2 M MgCl2
Strong Strength pH < 2 Formic acid HCl 10 mM Glycine/HCl H3PO4 pH > 10 NaOH pH > 10 25~50% ethylene glycol 4 M MgCl2 6M guanidinechloride