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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Methods Mol Biol. 2010;613:1–23. doi: 10.1007/978-1-60327-418-0_1

Table 4.

Binding affinity comparison of kinetic and steady–state analysis for DB293 binding to the AATT DNA.

Exp. ka (M−1 s−1) kd (s−1) KA (M−1) kt [RU/(Ms)] Chi2 (RU)2 ka×RUmax/kt
Kinetics 6.75×105 0.0155 4.35×107 1.56×1010 0.278 1.5×10−3
Steay-state 4.87×107 0.22