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Stimulation of [35S]GTPγS binding by VAC extracts and fractions in CHO-MOR (A) and CHO-DOR (B) cells. The activation of G proteins, indicative of receptor activation by agonist activities, was determined by the stimulation of [35S]GTPγS binding. Each data point represents the mean ± SEM of three replicates and is representative of three experiments. Emax: ** P<0.01, *** P<0.001 Methanol vs other groups; ## P<0.01, ### P<0.001 Petroleum Ether vs other groups; & P<0.05 EtOAc vs CHCl3.
