Fig. 3.

Analogs do not inhibit [³H]nicotine (top) or [³H]MLA (bottom) binding. Analog concentration response curves for inhibition of [³H]nicotine (NIC) and [³H]MLA binding to rat whole brain membranes are shown. Nonspecific binding was determined in the presence of 10 μM cytisine or 10 μM NIC for the [³H]NIC and [³H]MLA assays, respectively. Control represents [³H]NIC or [³H]MLA binding in the absence of analog (46.4 ± 4.20 and 49.1 ± 2.31 fmol/mg protein, respectively). Data are mean ± S.E.M. (n = 3–4 rats/compound).