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. 2010 Nov 22;120(12):4388–4398. doi: 10.1172/JCI32726

Figure 5. Blunted responses to Iso in RyR2-S2808A+/+ ventricular myocytes and SANCs.

Figure 5

(A) Representative Ca2+ transients and (C) fractional shortening traces from WT and RyR2-S2808A+/+ ventricular myocytes loaded with Fura-2 paced at 3 Hz in the absence and presence of 100 nM Iso. The label "Δ 0.5” refers to a 0.5 change in the Fura-2 (340:380) ratio. (B) Summary of Ca2+ transient and (D) fractional shortening data, demonstrating that the RyR2-S2808A+/+ myocytes have blunted increases in Ca2+ transient and fractional shortening amplitude in response to Iso (*P < 0.05). (E) Representative SAPs generated from WT and RyR2-S2808A+/+ SANCs in the absence (left) and presence of 50 nM Iso (right). (F) Bar graph summarizing the rate of SAP generation in WT (n = 9) versus RyR2-S2808A+/+ SANCs (n = 8; *P < 0.05).