Figure 4. Both LXR isoforms are required for maximal monocyte-derived cell emigration from plaques.

1 week prior to transplantation, circulating monocytes in Apoe^–/–, Nr1h3^–/–Apoe^–/–, and Nr1h2^–/–Apoe^–/– donor mice (n = 8 per group) were labeled with fluorescent beads as described in Methods. Pre-transplant (A) and 3 days after transplant, aortic arches (B) as well as iliac (C), hepatic (D), and brachial (not shown) LNs were harvested, and the contents of beads in the plaques and nodes were determined (see Methods). There were no beads recovered from the brachial nodes in any group. Note that there were no statistically significant differences in baseline bead number among the 3 donor groups (A). *P < 0.05 versus Apoe^–/–.