Figure 7. p300 overexpression impairs liver glucose homeostasis and leads to the development of glucose intolerance and insulin resistence.

Mice were injected with p300- and/or SIK2-overexpressing adenovirus and studied 7 days later in the fed sate. (A) Left: OGTT and ITT. Right: Postprandial blood glucose and insulin concentrations (n = 6 per group; *P < 0.01). (B) Relative Pepck and G6Pase gene expression (n = 6 per group; *P < 0.01). (C) NF-κB–luc reporter gene assay. HepG2 cells were transfected with a NF-κB–luc reporter vector and stimulated with 10 ng/ml TNF-α. Luminescence was measured 6 hours after TNF-α incubation. Data are representative of 3 independent experiments (mean ± SEM; *P < 0.05).