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. 2010 Nov 8;120(12):4289–4302. doi: 10.1172/JCI42015

Figure 1. Pit-1 regulates proliferation and cell death in the MCF-7 human breast adenocarcinoma cell line.

Figure 1

(A) Overexpression of Pit-1 in the MCF-7 cell line for 48 hours increases BrdU uptake, while knockdown of Pit-1 has the opposite effect. (B) Representative example of incorporation of BrdU in MCF-7 cells after overexpression or knockdown of Pit-1. For overexpression, the cells were immunostained with an anti-hemagglutinin antibody to localize Pit-1 expression. Scale bar: 50 μm. (C) Flow cytometric analysis of apoptosis in MCF-7 cells 48 hours after Pit-1 overexpression or knockdown. (D) Western blot shows cleavage of poly (ADP-ribose) polymerase (PARP) (89 kDa), which is used as an apoptosis indicator, 48 hours after transfecting MCF-7 cells with Pit-1 siRNA-1 and siRNA-2. (E and F) Western blot of cyclin D1, Bcl-2, Pit-1, and β-actin in MCF-7 cells 48 hours (E) after Pit-1 overexpression or (F) after Pit-1 knockdown. (D–F) The major 31- and 33-kDa immunoreactive bands corresponding to Pit-1 are indicated with double arrowheads. Values are reported as mean ± SD. **P < 0.01, ***P < 0.001.