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. 2010 Nov 8;120(12):4289–4302. doi: 10.1172/JCI42015

Figure 7. Pit-1, independently and through Snai1, modifies expression of proteins involved in EMT and cell motility.

Figure 7

(A) Western blots of E-cadherin, β-catenin, vimentin, Snai1, and Pit-1 in control MCF-7 cells 48 hours after transfection of pRSV-hPit-1 and/or Snai1 siRNA. (B) Immunohistochemistry of E-cadherin, β-catenin, and vimentin, as indicated in A. Scale bar: 25 μm. (C) Migration and invasion assay in MCF-7 control cells and cells transfected as described in A. (D) Western blots of β-catenin, vimentin, Snai1, and Pit-1, and β-actin for control MDA-MB-231 cells, as well as for cells 48 hours after (i) Snai1 overexpression, (ii) Pit-1 knockdown, and (iii) both Snai1 overexpression and Pit-1 knockdown. (E) Immunohistochemistry of E-cadherin, β-catenin, and vimentin, as described in D. Scale bar: 25 μm. (F) Migration and invasion assay in MDA-MB-231 control cells and cells transfected as described in D. Values are expressed as mean ± SD. ***P < 0.001 versus controls.