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. 2010 Nov 1;120(12):4569–4582. doi: 10.1172/JCI42869

Figure 3. RI-BPI–induced cell death is rescued by p300 and BAT3 inhibition.

Figure 3

(A) A panel of 7 BCL6-dependent DLBCL cell lines (OCI-Ly7, SU-DHL6, OCI-Ly1, Farage, OCI-Ly3, SU-DHL4, and OCI-Ly10) was exposed in triplicate to RI-BPI (10 μM) (dark gray bars), the p300-HAT inhibitor Lys-CoA (light gray bars), and the combination of both (black bars) for 48 hours (versus respective CPs). Cell viability (as percentage of CP-treated cells) is shown on the y axis. The experiment was carried out in triplicate with biological duplicates. (B) The BCL6-dependent SU-DHL4, SU-DHL6, and OCI-Ly3 cell lines were transfected with siRNA for BAT3 (siBAT3) or nontargeting sequence (siNT) and treated with RI-BPI (10 μM) or control. After 48 hours, viability was determined. Results are expressed as percentage of viable cells normalized to control (siNT). The experiment was carried out in 4 replicates with biological triplicates. Immunoblotting for BAT3 corresponding to the transfected cells is shown in Supplemental Figure 6. Data are presented as mean with 95% CI.