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. Author manuscript; available in PMC: 2010 Nov 29.
Published in final edited form as: Curr Biol. 2009 May 28;19(12):1012–1018. doi: 10.1016/j.cub.2009.05.018

Figure 1. CPAP is required for centriole duplication in cycling cells.

Figure 1

(A-D) U2-OS cells transfected with CPAP siRNAs for 72 h and stained for α-tubulin (green), Centrin-3 (red). In this and other figure panels, DNA is viewed in blue and insets show higher magnification views of denoted regions of interest. Two representative bipolar (A-B) and monopolar (C-D) figures are shown. (E-F) U2-OS cells treated with the indicated siRNAs for 72 h, a synchronized by a thymidine block for the last 24 h and stained with antibodies against Centrin-2 (green) and HsSAS-6 (red). 73% of cells treated with control siRNAs displayed HsSAS-6 signals near Centrin-foci (N=226), compared to 76% of cells treated with CPAP siRNA with a single Centrin focus (N=54).