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. Author manuscript; available in PMC: 2010 Nov 29.
Published in final edited form as: Hepatology. 2008 Dec;48(6):1942–1953. doi: 10.1002/hep.22541

Figure 1. Apoptotic morphology and annexin-V/PI staining of FasL-treated primary hepatocytes plated on collagen.

Figure 1

Figure 1

Figure 1

(A–D) Phase contrast and DNA fluorescence (Hoechst 33342) microscopy of untreated (A,C) and N2A FasL-treated (4 h, 50 ng/ml) (B,D) wt (A,B) and Bid−/− (C,D) primary mouse hepatocytes cultured on collagen. Magnifications are 200 × and 800 ×, respectively. (E) FACS analysis of GFP-annexin-V/PI stained wt, Bid−/−, Bak−/−, XIAP−/− primary mouse hepatocytes and wt 3T9 MEFs treated with 50 ng/mL N2A FasL for 0–10 h. The percentages of GFP-annexin-V/PI double negative cells are depicted. The values represent the means of 3 independent experiments ± s.d.