Table 2.
Comparison of assays for the effect of chloroquine (CQ) on ΔΨm in Plasmodium falciparum.
| Plasmodium falciparum strain | Chloroquine concentration | Chloroquine incubation time | Probe | Affect on ΔΨm | Reference |
|---|---|---|---|---|---|
| F32 (CQ sensitive) | 20 nM | 20 min | DiOC6(3) | No effect | Nyakeriga et al. [39] |
| 3D7 (CQ sensitive) 7G8 (CQ resistant) |
55 nM 1400 nM |
18 h 18 h |
JC-1 JC-1 |
Sig. loss Not sig. |
Meslin et al. [14] |
| 3D7 (CQ sensitive) 7G8 (CQ resistant) K1 (CQ resistant) |
>3 μM >3 μM >12 μM |
4 h >8 h >8 h |
JC-1 JC-1 JC-1 |
Sig. loss Sig. loss Sig. loss |
Ch'ng et al. [37] |
All assays were performed in red blood cell stages. Higher doses and longer incubation times altered the effect of the drug on loss of mitochondrial transmembrane potential (ΔΨm) and different strains of parasite reacted differently.