Figure 2.

Increased levels of ferritin polypeptides in HF skin fibroblasts. a) Representative western blot showing the expression of ferritin subunits in non-HF and HF fibroblasts. Western blot was carried out using antibodies against ferritin heavy chain (FTH), the amino terminus of ferritin light chain (FTL) that recognizes wild-type and mutant FTL subunits, and abs specific for the mutant subunit (MT-FTL). b) Densitometric analysis of ferritin subunit protein levels shows increased levels of FTH and wild-type and mutant FTL. Results are the means and SD of three independent experiments (*p < 0.05).