Figure 6.

E-cadherin and N-cadherin have opposite effects on gap junction assembly. Myc-tagged N-Cad and E-Cad were introduced, respectively, into RL-EΔN and RL-NΔE cells as described in Materials and Methods. Pooled polyclonal cultures were immunostained for Cx43 (green) and either endogenous cadherin or Myc (red). Note that E-Cad expression (+E-Cad) in RL-NΔE cells restores GJ formation (A) and N-Cad expression (+N-Cad) in RL-EΔN cells partially disrupts GJ assembly (B) and induces intracellular accumulation of Cx43. Bar = 20 μm.