Table 2.

 Effect of E-cadherin and N-cadherin expression on intracellular accumulation of connexin43 in RL-EΔN and RL-NΔE cells

Exp. #	RL-EΔN	RL-EΔN +N-Cadherin	RL-NΔE	RL-NΔE +E-Cadherin
1	5 ± 2	14 ± 6	24 ± 7	10 ± 4
2	7 ± 3	23 ± 7	33 ± 9	15 ± 5
3	4 ± 2	27 ± 9	37 ± 11	17 ± 11

N-cadherin and E-cadherin were introduced, respectively, into RL-EΔN and RL-NΔE cells (see Materials and Methods). Pooled polyclonal cultures were immunostained for Cx43 and E-cadherin and N-cadherin. Images were captured using 0.5-μm Z-steps and deconvolved. After merging all the deconvolved image stacks into a single plane, the number of intracellular puncta were counted in 10 randomly selected cells. E-cadherin or N-cadherin immunostaining was used to delineate cell boundaries. The mean number of puncta per cell ± SE was then calculated. Note that N-cadherin expression in RL-EΔN cells increased the number of intracellular puncta, whereas expression of E-cadherin in RL-NΔE cells had the opposite effect.