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. 2010 Dec 1;21(23):4108–4119. doi: 10.1091/mbc.E10-07-0580

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 2. — β1A, and not β3, integrin is essential for invadosome formation. (A) β3 is not essential for invadosome formation and self-assembly into rosettes, visualized by F-actin (in red in merge) and phospho-Y397-FAK (in blue in merge) staining, which occurs in both MEF-SrcYF β3 +/+ or −/− cells. (B) In contrast, β1 depletion in MEF-SrcYF β1^LoxP/LoxP expressing the CRE recombinase for 96 h resulted in the disappearance of isolated invadosomes or in rosettes probed by phalloidin staining. (C) Quantification of the percentage of cells forming invadosomes reveals that almost 95% of MEF-SrcYF β1^LoxP/LoxP treated with CRE recombinase did not form this structure 4 d after infection (n = 650 counted cells/condition). (D) Quantification by qPCR shows an average decrease of 95% in the level of β1 mRNA 96 h post-CRE treatment. Bars, 3 μm (A) and 10 μm (B).