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. 2010 Dec 1;21(23):4130–4140. doi: 10.1091/mbc.E10-01-0022

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 3. — ERGIC tubules induced by Arf1+Arf4 double knockdown are sensitive to some PLA₂ antagonists. Cells were processed as described in Figure 1D and were either untreated (A) or treated for 20 min with ONO-RS-082 (B), pyrrolidine (C), BEL (D), or BEL supplemented with LPC (E). Bar, 5 μm. Insets are magnifications of the highlighted regions. (F) 100% represents the total number of cells counted. Results are shown as mean ± SD of three to nine independent experiments. p values were calculated with a Wilcoxon rank test. The white bar represents pSUPER-transfected cells. Black bars represent Arf1+Arf4 knockdown cells.