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. 2010 Dec 1;21(23):4184–4196. doi: 10.1091/mbc.E10-02-0105

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 8. — 7SK RNA depletion affects the displacement of Cdk9 from the reporter gene locus during transcriptional silencing. Immunolocalization of CDK9 (Ab; Aa–Ja) in U2OS 2-6-3 tet-OFF cells demonstrates that in the majority of control oligo treated cells (A–E), CDK9 is displaced within 10–15 min post DOX addition (Ca–c). However, in cells that are depleted of 7SK RNA (F–J) CDK9 continues to associate with the gene locus for a longer duration of time post-DOX addition (H–I). DNA is counterstained with DAPI (Ac–Jc). The bar represents 5 μm. (K) Histogram showing reduced displacement of CDK9 from the gene locus in cells that are transfected with ASO against 7SK RNA (n = 50). Scale bar represents 5 μm.