Figure 5.

(a) Bright field microscopy image of a zebrafish embryo shows the gold NP injection area (white circle) and several numbered irradiation points where single pump laser pulses were applied; (b) time-resolved scattering differential images of three numbered points obtained with the 10 ns delayed (relative to the pump laser pulse) pulsed probe laser (690 nm, 0.5 ns), the white circle shows the aperture of the pump laser beam; (c) profiles of the pixel image amplitudes for the corresponding images in (b) (1 μm is 14 pixels); (d) corresponding time response obtained during the exposure to the pump laser pulse shows the bubble expansion and collapse, the signal after the bubble collapse is identical to the baseline, note the different time (X) and output signal (Y) scales.