Table 1. a) N-terminal protein sequencing result for the unknown 75 kDa serum protein and b) sequence of primers used for cloning.

For protein sequencing lower case letters denote the probable amino acid at that position and×denotes the residue could not be determined. Residues underlined and in bold denote potential N-linked glycosylation site.

Protein	N-terminal protein sequence
Gc p75	FPwLKHQPNITEDELNxxh
Primer name	Sequence
GcP75-f1	5’-CAYCARCCiAAYATHACiGARG-3’
GcP75-f2	5’-CACTCGCTTCTATCCTCACTGGG-3’
GcP75-f3	5’-GAAGTTTCTGCTGGGAGGATTGTG-3’
GcP75-r1	5’-CTGGGGTGGGAGTGATGCTG-3’
GcP75-r3	5’-GTACACAAGACATGAAATCTGCGCG-3’
LeHx-f1	5’-ATGAAGTCCCTGCTGGGAGGAGTG-3’
LeHx-r1	5’-CGAGCCACCGCCTTGTAGATGAAC-3’
LeHx-f4	5’-GTTCATCTACAAGGCGGTGGCTCG-3’