Table 2. Summary of putative C. elegans O-GlcNAc targets.
| Proteins | Molecular Identity | Expression in daf-2 mutant | O-GlcNAc modified in vertebrate homologs |
|---|---|---|---|
| PHI-37 | F0F1-type ATP synthase | Downregulated [46] | NR |
| ATP-2 | F0F1-type ATP synthase | Downregulated [46] | NR |
| MDH-1 | Malate dehydrogenase | Downregulated [47] | MDH1 [7] |
| GPD-2, GPD-3 | Glyceraldehyde-3-phosphate dehydrogenase, major isozyme | Upregulated [48,49] | GAPDH [7,50] |
| GPD-1, GPD-4 | Glyceraldehyde-3-phosphate dehydrogenase, minor isozyme | Downregulated [46] | GAPDH [7,50] |
| EFT-3, EFT-4 | Translation elongation factor 1 alpha | Downregulated [46] | EEF1AO [50] |
| ACT-1, ACT-2, ACT-3, ACT-4 | Actin | Downregulated [46] | ACTG1 [7] |
| TBA-1, TBA-2, TBA-4 | Alpha tubulin | Downregulated [46] | TUBA1A [7] |
| VIT-6 | Vitellogenin | Downregulated [46] | NR |
| VHA-13 | Vacuolar protein translocating ATPase | NR | NR |
| CDC-48.2 | AAA-type ATPase | NR | NR |
| F27D4.1 | Electron transfer flavoprotein | NR | NR |
| C16A3.10 | Ornithine aminotransferase | Downregulated [48] | NR |
EFT-3 and EFT-4 have identical protein sequences, as do GPD-2 and GPD-3. The peptides identified by LC-MS/MS did not distinguish between the almost identical ACT-1, ACT-2, ACT-3, and ACT-4; TBA-1, TBA-2, and TBA-4; or GPD-2 and GPD-3, respectively. NR, not reported.