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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Mol Immunol. 2010 Sep 1;48(1-3):219–230. doi: 10.1016/j.molimm.2010.08.007

Figure 5.

Figure 5

C3a and C5a induce cytokine and chemokine production in mixed glia and BV-2 microglia. (A) Mixed glia were treated with 50nM C3a, 50nM C5a or a combination of 50nM C3a and C5a for 24 hours. After 24 hours of treatment, supernatants were collected and used in ELISAs specific for IL-6 or CCL4 (B) BV-2 microglia were treated with 50nM C3a, 50nM C5a or a combination of 50nM C3a and C5a for 72 hours. After 72 hours of treatment, supernatants were collected and used in an ELISA specific for CCL4. (C) Primary astrocytes were stimulated with 50nM C3a, 50nM C5a or a combination of 50nM C3a and C5a. After 144 hours, cell supernatants were collected from the cell cultures and analyzed using an ELISA specific for CCL4. Samples from three experiments were used for ELISAs. Statistical significance was calculated using unpaired student’s t-test, * p < 0.05. Error bars represent standard deviation.