Figure 3.
SelP protects HEK-293 cells from oxidation by the pharmacological addition of 15-HpETE. A) Time course oxidative changes measured by DPPP fluorescence following 30 μM 15-HpETE addition in cells with standard media (blank, square), 100 nM sodium selenite (NaSeO3, circle), concentrated control media ((−) SelP, up triangle), and SelP enriched media ((+) SelP, down triangle). The (+) SelP condition is statistically different at all time points (p<0.001). B) Dose response of 15-HpETE oxidative changes measured by DPPP fluorescence (right), as well as 15-HETE at 30 μM (left). The (+) SelP condition is significantly different from the other conditions at 10 to100 μM 15-HpETE (***, p<0.001).