Fig. 3.

Representative FACS analysis is shown for purified CD34+ cells or MNCs from UCB in 3D culture system. Q1 quadrant hint CD34⁺/CD38⁻ cell fragment, Q1 and Q2 quadrants hint CD34⁺ cells at onset (a, d). CD34⁺/CD38⁻ cells account for the highest amount of CD34⁺ cells after 2 weeks in culture of CD34⁺ cells (b). CD 34⁺ and CD34⁺/CD38⁻ cells were increased in MNC after 2 weeks in culture (e). The CD34⁺/CD38⁻ cells were increased after 5 weeks in culture of CD34⁺ cells (c). Both CD 34⁺ and CD34⁺/CD38⁻ cells in MNC were increased after 5 week in culture (f)