Table 1.

Yield of CD34⁺ cells and CD34⁺/CD38⁻ cells cultured in the biomimetic osteoblast niche

Inplanting cells	Initial input	2-weeks culture	5-weeks culture
CD34+ Cells
CD34+	2 × 104	7.5 ± 1.2 × 104*	1.7 ± 0.5 × 104
CD34+/CD38−	1.3 ± 0.3 × 103	5.7 ± 0.7 × 104*	1.0 ± 0.5 × 104*
MNC
CD34+	1.3 ± 0.6 × 103	6.8 ± 2.3 × 103*	2.1 ± 0.4 × 103*
CD34+/CD38−	1.5 ± 0.1 × 102	5.3 ± 2.1 × 103*	1.1 ± 0.3 × 103*

Note: 2 × 10⁴ UCB CD34⁺ cells or 1 × 10⁵ MNCs were seeded in the biomimetic osteoblast niche. After 2–5 weeks in culture, CD34⁺ cells and CD34⁺/CD38⁻ cells were evaluated by FCM. (*) Significant difference between each time point and the input (p < 0.05)