Fig. 3.

The transcriptional and proteic expression of melanoma-associated antigens in melanoma cell cultures established in different growth media. a The transcriptional expression of tumor antigens was determined by semi-quantitative RT–PCR. Normalized scores were determined by comparison with the positive and negative controls expressed as a percentage. b The expression of intracellular tumor antigens was determined by FACS analysis. Normalized scores were calculated by subtracting the number of cells stained for the relevant Ag by the background of cells stained by the relevant isotype control mAb. Normalized scores for (a) and (b) were represented by: