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. Author manuscript; available in PMC: 2010 Nov 30.
Published in final edited form as: Mol Microbiol. 2008 Oct 24;70(6):1378–1396. doi: 10.1111/j.1365-2958.2008.06487.x

Figure 1.

Figure 1

VjbR activates the promoter upstream of virB1 in E. coli.(A) Top: Western blot using anti-His antibodies showing expression of VjbR-His6 or BAB1_0345-His6 after induction with IPTG and showing that expression of VjbR-His6 is not reduced after addition of C12-HSL. Bottom: β-galactosidase activity of E. coli with pSURS2 was measured after no induction (white bars) or induction with IPTG (black bars) of VjbR-His6 and a negative control BAB1_0345-His6. Hatched bars indicate induction with IPTG and the addition of 30 µM or 60 µM C12-HSL to the culture. Values are the averages ± standard deviations of duplicate samples from a representative experiment that was repeated at least three times independently. (B) β-galactosidase activity of E. coli with empty pSURS1 vector and expressing VjbR-His6 or BAB1_0345-His6.