(A) Cytotoxicity assay showing % LDH release at 11 hours post infection from J774 macrophages infected at MOI 1:500 with either wild type or virB2 mutant Brucella abortus expressing TEM1-GST or TEM1-effector fusions. (B) Cytotoxicity assay showing % LDH release at 5, 8 and 11 hours post infection from J774 macrophages infected at MOI 1:500 with either wild type or virB2 mutant Brucella abortus expressing TEM1∷VceC. Results shown in A and B are the mean ± SD of three independent experiments. For statistical analysis and calculation of SD, data were analysed using a Student’s t test. ***, p<0.001; **, p<0.01; *, p<0.05.