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. Author manuscript; available in PMC: 2010 Nov 30.
Published in final edited form as: Mol Microbiol. 2008 Oct 24;70(6):1378–1396. doi: 10.1111/j.1365-2958.2008.06487.x

Figure 13.

Figure 13

VjbR-dependent activation of virB and vceC expression in B. abortus at different time points after switching from TSB to modified minimal E-medium, pH 5. At time points 0 h and 7 h after switching media, β-galactosidase activity was measured in samples of B. abortus wild type and vjbR mutant strains containing a chromosomal virB1∷lacZ fusion (A) or vceC∷lacZ fusion (B). C. Western blot using anti-VirB8 antiserum shows protein levels of VirB8 in wild type and vjbR mutant B. abortus in samples taken at several time points after switching to modified minimal E-medium at pH 5. In each lane, the total protein equivalent to 1 × 108 CFU was loaded, as determined by measurement of OD600 for each culture.