Fig. 3.

Silver-stained two-dimensional gel of proteins extracted from Medicago sativa leaves grown under control (A) and drought (B) conditions. In the first dimension, 125 mg of total protein was loaded on a 18 cm IEF strip with a linear gradient of pH 4–7. The second dimension was conducted in 12% polyacrylamide (w/v) gels (20×20 cm) (for details, see Materials and methods). The gel image analyses conducted with Progenesis SameSpots software v3.0 and the subsequent mass spectrometry analyses identified up to 26 proteins (marked by arrows) that, statistically, were involved in the plant response to drought.