Table 2.
Annotation of up/down-regulated identified spots following drought in silver stained two-dimensional electrophoresis gels of leaves
Spot no. represents the number of proteins assigned. Spot volume (%) is an estimation of relative protein abundance. The pI and molecular mass (Mr) values shown are the theoretical and experimental values. SC represents the protein sequence coverage (%) score, which is the Mascot score of the in-solution digestion protocol. Function, the predicted protein function is assigned according to the NCBInr-protein sequence database.
| Spot no. | Spot % volume variations | Experimental pI/Mr | Theoretical pI/Mr | PM | SC (%) | Score (P <0.05 corresponding to score >51) | Protein name/organism/NCBI accession no. | Regulation |
| 01. Metabolism | ||||||||
| 159 | 641.02 | 6.31/51.0 | 6.06/49.71 | 5 | 17 | 198 | Dihydrolipoamide dehydrogenase/Pisum sativum/gi|9955321 | Up-regulated |
| 172 | 720.36 | 5.35/40.7 | 6.29/47.08 | 14 | 19 | 466 | Glutamine synthetase/Medicago sativa /gi|17367236 | Up-regulated |
| 02. Energy | ||||||||
| 8 | 170.34 | 6.85/47.8 | 9.75/49.63 | 2 | 28 | 62 | Glyceraldehyde 3-phosphate dehydrogenase/Ficus wassa/gi|87300266 | Up-regulated |
| 10 | 16.92 | 4.85/56.5 | 4.99/61.78 | 2 | 4 | 153 | β subunit of mitochondrial ATP synthase/Chlamydomonas reinhardtii /gi|159466892 | Down-regulated |
| 12 | 16.84 | 4.77/52.8 | 5.25/52.68 | 2 | 5 | 60 | β subunit of ATP synthase/Eurya sp./gi|20269410 | Down-regulated |
| 15 | 167.44 | 7.19/46.0 | 8.93/43.411 | 5 | 20 | 218 | (NADP-dependent glyceraldehydephosphate) Glyceraldehyde-3-phosphate dehydrogenase A/Pisum sativum/gi|120658 | Up-regulated |
| 40 | 16.33 | 6.34/63.0 | 6.09/52.11 | 3 | 8 | 138 | Rubisco large subunit/Pieris phillyreifolia /gi|1352804 | Down-regulated |
| 42 | 16.29 | 6.30/62.1 | 6.0/51.00 | 4 | 10 | 159 | Rubisco large subunit/Heisteria cauliflora/gi|112408850 | Down-regulated |
| 80 | 155.37 | 6.37/41.5 | 5.92/35.47 | 5 | 21 | 191 | Cytosolic malate dehydrogenase/Cicer arietinum/gi|10334493 | Up-regulated |
| 97 | 48.84 | 5.99/35.7 | 5.83/38.63 | 5 | 18 | 258 | Fructose-bisphosphate aldolase 1/Pisum sativum/gi|399024 | Down-regulated |
| 102 | 15.01 | 6.30/62.1 | 6.22/51.85 | 6 | 14 | 233 | Rubisco large subunit/Akania bidwillii/gi|2500654 | Down-regulated |
| 135 | 57.1 | 6.70/51.0 | 6.73/29.96 | 2 | 10 | 60 | Rubisco large subunit/Echeandia sp./gi|1865802 | Down-regulated |
| 152 | 14.01 | 5.04/35.2 | 5.63/29.99 | 37 | 65 | 1057 | Rubisco activase/Medicago sativa/gi|23320705 | Down-regulated |
| 155 | 1381.95 | 7.02/34.0 | 8.80/43.31 | 14 | 24 | 607 | Glyceraldehyde-3-phosphate dehydrogenase A, chloroplast precursor/Pisum sativum/gi|120658 | Up-regulated |
| 160 | 13.37 | 4.20/30.5 | 5.83/42.21 | 10 | 19 | 306 | Sedoheptulose-1,7-bisphosphatase precursor/Oryza sativa (indica cultivar-group)/gi|27804768 | Down-regulated |
| 176 | 24.45 | 5.05/30.7 | 5.41/39.00 | 3 | 11 | 73 | Phosphoribulokinase/Pisum sativum/gi|1885326 | Down-regulated |
| 178 | 76.01 | 6.17/27.2 | 8.23/30.34 | 3 | 9 | 107 | Ribulose-phosphate 3-epimerase/Spinacia oleracea/gi|2833386 | Down-regulated |
| 06. Protein destination and storage | ||||||||
| 78 | 155.68 | 4.86/33.9 | 8.80/43.31 | 11 | 35 | 514 | 14-3-3-like protein/Cicer arietinum/gi|148612111 | Up-regulated |
| 84 | 15.47 | 4.85/62.5 | 5.20/61.18 | 21 | 35 | 985 | Putative rubisco subunit binding-protein alpha subunit/Trifolium pratense/gi|84468288 | Down-regulated |
| 110 | 148.88 | 4.61/40.0 | 4.60/38.37 | 5 | 11 | 178 | Plastoglobulin-1/Pisum sativum/gi|62900628 | Up-regulated |
| 126 | 145.18 | 6.31/27.5 | 6.30/24.60 | 1 | 8 | 69 | Proteasome subunit beta type-1 (20S proteasome alpha subunit F) (20S proteasome subunit beta-6)/Petunia×hybrida/ gi|17380185 | Up-regulated |
| 07. Transport | ||||||||
| 9 | 169.45 | 6.33/46.5 | 6.04/35.10 | 2 | 9 | 62 | Putative chloroplast inner envelope protein/Oryza sativa/gi|15341602 | Up-regulated |
| 09. Cell structure | ||||||||
| 1 | 178.63 | 5.95/40.2 | 6.01/40.82 | 3 | 11 | 149 | Reversibly glycosylated polypeptide/Gossypium hirsutum/gi|18077708 | Up-regulated |
| 11. Disease/defence | ||||||||
| 25 | 161.22 | 4.96/24.9 | 4.93/21.84 | 4 | 24 | 211 | 2-cys peroxiredoxin-like protein/Hyacinthus orientalis/gi|47027073 | Up-regulated |
| 28 | 165.27 | 5.49/25.0 | 5.47/24.04 | 2 | 8 | 111 | Dehydroascorbate reductase/Zinnia elegans/gi|50058092 | Up-regulated |
| 68 | 158.47 | 6.45/25.6 | 7.16/26.62 | 1 | 6 | 67 | Superoxide dismutase/Medicago sativa/gi|23534609 | Up-regulated |
| 78 | 155.68 | 4.86/33.9 | 8.80/43.31 | 11 | 35 | 514 | 14-3-3-like protein/Cicer arietinum/gi|148612111 | Up-regulated |
| 155 | 1381.95 | 7.02/34.0 | 8.80/43.31 | 14 | 24 | 607 | Glyceraldehyde-3-phosphate dehydrogenase A, subunit/Pisum sativum/gi|120658 | Up-regulated |
| 159 | 641.02 | 6.31/51.0 | 6.06/49.71 | 5 | 17 | 198 | Dihydrolipoamide dehydrogenase/Pisum sativum/gi|9955321 | Up-regulated |