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Location and consequences of T-DNA insertion in the dda1-1 mutant. (A) The genomic structure of DDA1 indicating the position of the T-DNA insertion in dda1-1 (triangle). (B) RT-PCR analysis of DDA1 transcript levels in 7-day-old seedlings of Col and dda1-1. RT-PCR products were detected by blotting and probing with gene-specific probes, following either 15 cycles (DDA1) or 10 cycles (ACT2) of amplification. The primers used for DDA1 amplification span the entire coding region.
