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. 2010 Aug 25;62(1):221–233. doi: 10.1093/jxb/erq259

Fig. 4.

Fig. 4.

DDA1 transcript levels are regulated by AXR3 and HY5. (A) RT-PCR analysis of DDA1 transcript levels in 7-day-old axr3-1 mutant seedlings following 0 h or 2 h exposure to 20 μM IAA. (B) RT-PCR analysis of DDA1 transcript levels in 7-day-old Ler and hy5-1 seedlings. RT-PCR products were detected by blotting and probing with gene-specific probes, following either 15 cycles (DDA1) or 10 cycles (ACT2) of amplification.