Table 1.
Changes of ascorbate peroxidase (APX), catalase (CAT) activities, and hydrogen peroxide (H2O2) content
| Treatment | APX (U layer−1) |
CAT (U layer−1) |
H2O2 (μmol g−1 FW) |
|||
| ZnPPIX→ | 12 h | 48 h | 12 h | 48 h | 12 h | 48 h |
| ABA | 5.60±0.61 a | 4.80±0.91 a | 19.1±1.50 a | 7.88±0.83 a | 0.54±0.16 d | 0.68±0.04 d |
| GA | 2.02±0.18 c | 0.16±0.02 c | 10.2±0.85 c | 0.60±0.13 d | 0.93±0.13 a | 1.57±0.15 a |
| GA+Ht | 4.17±0.32 b | 1.69±0.44 b | 12.6±1.54 b | 3.95±0.34 b | 0.59±0.24 cd | 0.94±0.06 c |
| GA+CO | 3.18±1.43 bc | 1.44±0.24 b | 13.9±1.07 b | 2.67±0.78 c | 0.75±0.05 bc | 1.23±0.07 b |
| GA+Fe2+ | 3.35±0.57 bc | 1.11±0.33 bc | 2.56±0.44d | 0.49±0.12 d | 0.84±0.24 ab | 1.43±0.06 a |
| GA+BR | 4.01±0.46 b | 1.46±0.28 b | 13.1±0.57 b | 3.44±0.45 bc | 0.66±0.05 cd | 0.97±0.07 c |
Layers pretreated with 100 μM ZnPPIX for 6 h were further incubated in a medium containing 5 mM CaCl2 and 50 μM ABA or GA alone, or GA plus 10 μM haematin (Ht), 1.0% saturation of CO aqueous solution, 10 μM each of FeSO4 (Fe2+) or BR for another 12 h and 48 h. Data are means ±SD of at least four independent samples. Different letters within columns are significantly different at P <0.05, according to Duncan's multiple test.