FIGURE 3.
Epitope-mapping of the Phl p 5-specific IgG1 response in immunized mice. (A) Schematic representation of Lol p 5A and Lol p 5B fragments expressed by the phage clones. (B) Lol p 5A- and Lol p 5B-derived clones (11-120) and a phage clone without insert (λgt11) were immobilized to a nitrocellulose filter in the order given and probed with mouse sera from groups A, D, and G obtained before sensitization (lane 1), on day 21 (lane 2), and on day 42 (lane 3).