Table 1. Mouse groups for IN immunization.

Groups*	Dose**
VLPs	10 µg VLPs
cVLPs***	10 µg VLPs
VLPs + sFliC****	10 µg VLPs+0.5 µg sFliC
M1 only VLPs	10 µg VLPs

*Naïve mice and a PBS immunized group did not show antigen specific responses and thus are not presented here.

**Doses were given in a total of 25 µl split between two nostrils.

***The dose of cVLPs was normalized to contain an HA content equal to 10 µg of standard VLPs by Western Blot. The content of the flagellin protein was 0.5 µg in each dose of cVLPs calculated by using purified recombinant flagellin as a standard.

****Purified recombinant soluble flagellin (sFliC) was mixed with standard VLPs before administration.