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. 2010 Oct 31;10(5):173–180. doi: 10.4110/in.2010.10.5.173

Figure 2.

Figure 2

THP-1 cells transfected with BAFF-specific siRNA were stimulated with agents that can interact with APRIL. (A) THP-1 cells transfected with control siRNA (Control) or BAFF specific siRNA (siBAFF) were stimulated with 1µg/ml of LPS, TACI:Fc (TF), BCMA:Fc (BF), human IgG (H), anti-APIRL mAb (A), anti-BAFF mAb (B), or mouse IgG. Culture supernatants were collected 24 hr after the activation and the concentration of IL-8 was measured using double sandwich ELISA. IL-8 concentrations were compared with anti-APRIL stimulated samples which were set to 100%. (B) THP-1 cells transfected with siBAFF was stimulated with 1µg/ml LPS or 1, 3, 10µg/ml of BCMA:Fc for 24 hr. Culture supernatants were then collected for the measurement of IL-8 concentration using ELISA and for the analysis of MMP-9 activity through gelatin zymogram. Data points are represented as mean±SD of triplicate measurements. These experiments were repeated three times with essentially the same results. *p<0.001 when compared with negative control (the first lane). †p<0.001 when compared with the value obtained without T/B pre-incubation.