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. 2010 Nov 29;5(11):e15055. doi: 10.1371/journal.pone.0015055

Figure 1. Myf5-Cre mediated mutation of paternal Dlk1(cKO) results in defects in muscle formation and growth.

Figure 1

Asterisks in all graphs denote p<0.05compared to wild-type (WT) controls. A: Quantitative PCR confirming muscle-specific Dlk1 knockout in cKO mice in whole muscle, myofibers, myoblasts and BAT. B: Relative body weight (BW) of WT (n = 17, 9, 4 for all age, 2–4 month and 10–12 month mice, respectively) and cKO (n = 18, 9, 5) littermates. C: Relative mass of BAT of WT (n = 3) and cKO (n = 3) mice. D–K: Muscle fiber composition in representative fast-twitch (EDL) and slow-twitch soleus (SOL) muscles revealed by MyHC isoform-specific antibodies and qPCR. D: MyHC isoform staining of representative EDL. Total myofiber number for EDL(E) and SOL (I) muscle in WT and cKO (n = 4 pairs). Myosin heavy chain (MyHC) IIB gene expression in EDL (F) and SOL (J;n = 3 pairs) muscles. Percent of each MyHC isoform by immunostaining in EDL (G) and SOL (K; n = 3 pairs).