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. 2010 Nov 29;5(11):e15055. doi: 10.1371/journal.pone.0015055

Figure 4. Dlk1 regulates satellite cell self-renewal and differentiation.

Figure 4

A–D: Pax7 and MyoD expression in a representative cluster of myoblasts on a wild-type EDL fiber after 3 days of culture. Pax7+/MyoD−, Pax7+/MyoD+ and Pax7−/MyoD+ cells represent self-renewing, proliferating and differentiating cells, respectively. E–H: A representative cluster of myoblasts on a cKO fiber cultured under identical culture conditions. I: The average number of cells per cluster. J: The percentage of cells expressing Pax7 and MyoD (note that most cells co-express Pax7 and MyoD; see K below). K: The proportion of cells at three different statuses: self-renewal (S–R; Pax7+/MyoD−), differentiation (Diff; Pax7−/MyoD+) and proliferation (Prolif; Pax7+/MyoD+). In I–K, n = 34 for WT, and n = 27 for cKO; * indicates p<0.05 compared to WT.