Figure 6. Dlk1 up-regulation correlates to myogenic differentiation and skeletal muscle regeneration after injury.

A: Representative Western-blot image showing that Dlk1 protein levels increase 5 days after serum-withdrawal induced differentiation of C2C12 myoblasts. B: The relative level of Dlk1 mRNA in the TA muscle revealed by quantitative Realtime PCR 5 days after cardiotoxin-induced regeneration (* indicates P<0.05; n = 18 for control TA; 6 for CTX treated TA). C: Dlk1 protein expression revealed by Western-blot in resting and regenerating TA muscles 5 days after cardiotoxin treatment. D: A model depicting how Dlk1 level varies during muscle quiescence and regeneration, which correlates to suppression of NK-κB activity and the onset of myoblast differentiation.