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. 2010 Nov 29;5(11):e15513. doi: 10.1371/journal.pone.0015513

Figure 2. Prediction and confirmation of the target genes predicted for both let-7a (unedited) and let-7a-5C (edited).

Figure 2

(A) The confirmation of the target genes (Klf9, Gatm and Dnajb9) of let-7a in mouse 3T3 cells using dual-luciferase assay. Cells were co-transfected with negative control, let-7a (Pre-let-7a) or let-7a-5C (Pre-let-7a-5C) precursor, respectively; (B) The confirmation of the target genes (Tmem55a, Timp3 and Smad7) of let-7a-5C in mouse 3T3 cells; (C) The relative mRNA expression level of the three target genes of edited let-7a-5C in cultured mouse stromal cells transfected with negative control, Pre-let-7a or Pre-let-7a-5C; (D) Protein level of Smad7 was detected by Western blot after mouse stromal cells were transfected with negative control, Pre-let-7a or Pre-let-7a-5C; (E) Quantification of Smad7 protein expression in (D). The difference between Pre-let-7a and Pre-let-7a-5C was compared using t-test, and the significant difference between two groups was labeled with asterisk.