Figure 2. In vivo study protocol in pigtailed macaques.

(A) Two studies were performed. The first included five macaques, in which each monkey received placebo films followed six weeks later with RC-101 films. The second study included the five original macaques plus an additional monkey, in which half received RC-101 films and half received placebo films. Six weeks later, each monkey receiving the opposite film type. (B) Baseline measurements for colposcopy, vaginal pH, and microflora were obtained at day 0. At days 1–4, RC-101- or placebo-containing films were instilled intravaginally. At days 5 and 8, cytobrushes, blood, and pinch biopsies were obtained, and at day 8 CVL fluids were obtained from each monkey.