B.
| Fraction | Specific activity | |
|---|---|---|
| phenol hydroxylase | catechol 1,2-dioxygenase | |
| (nmol phenol/min/mg) | (nmol catechol/min/mg) | |
| Cytosol | 22.1 | not measured |
| Eluate, DEAE-Sepharose | 28.4 | 23.1 |
| Lyophilisate | not measured | not measured |
| Eluate, Sephadex G-100 | 14.8 | 54.8 |
| Eluate, Sephacryl S-300 | 45.9 | 100.0 |
| Eluate, Sepharose 4B | 30.0 | 110.8 |
Experimental conditions are described in the Material and methods section.