Figure 6.

TSC2 restricts 4E-BP1 repressor inactivation and viral replication in cells infected with a Us3-deficient virus. (A) NHDFs treated with nonsilencing (NS) or TSC2-specific siRNAs were either mock-infected or infected (MOI = 5) with Us3-deficient (ΔUs3) or wild-type (WT) HSV-1. After 15 h, total protein was harvested, fractionated by SDS-PAGE, and analyzed by immunoblotting with the indicated antisera. Phospho-4E-BP1 forms were resolved (hyper vs. hypo) as described in Figure 2A. (B) NHDFs treated with siRNAs as in A were infected (MOI = 1 × 10⁻⁴) with the indicated viruses. After 4 d, the virus produced was quantified by plaque assay in Vero cells. (Right panel) Immunoblot illustrating efficiency and persistence of TSC2 siRNA depletion after 4 d.