Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Dec 1;24(23):2654–2665. doi: 10.1101/gad.1989110

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2010 by Cold Spring Harbor Laboratory Press

Freely available online through the Genes & Development Open Access option.

PMC Copyright notice

Figure 3. — DLG5 regulates cell migration. (A) Immunoblot analysis of DLG5 knockdown in MCF10A cells using two independent shRNAs. (B) DLG5 knockdown in MCF10A cells leads to induction of cell migration as assayed by Boyden chambers. Numbers in the bottom right of each panel represent quantitation of the migration phenotypes and are derived by counting individual cells in three representative fields. (C) DLG5 knockdown in MCF10A cells leads to induction of cell migration, as assessed by wound scratch assays. (D) Immunoblot analysis showing DLG5 overexpression in MDA-MB-231 cells and its relative level in comparison with MCF10A cells. (E) DLG5 overexpression in MDA-MB-231 cells results in inhibition of cell migration. Numbers in the bottom right of each panel represent quantitation of the migration phenotypes and are derived by counting individual cells in three representative fields. (F) The phospho-specific antibody array identifies activation of multiple signaling pathways upon DLG5 knockdown using the best available hairpin.